Promega Klenow Fragment, DNA Polymerase I

Description

A DNA-dependent DNA polymerase that lacks the 5'→3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'→3' polymerase, 3'→5' exonuclease and strand displacement activities.

• DNA-Dependent DNA Polymerase that Lacks the 5′→3′ Exonuclease Activity
• Use in a variety of molecular applications
• Heat-inactivate by heating at 75°C for 10 minutes
• Active in many Promega 1X restriction enzyme buffers
• Supplied at a concentration of 5u/μL

†DNA polymerase kit that includes 5μmoles each of dATP, dCTP, dGTP, and dTTP at 10mM in water.

Specifications

• Concentration: 5 U/μL
• Color: Colorless
• Components: 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
• Purity: >80%
• pH: 7.2
• For Use With (Application): Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Recombinant E. coli strain
• Quantity: 150 U
• Product Type: DNA Polymerase I Large (Klenow) Fragment
• Molecular Weight (g/mol): 68
• Form: Liquid