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Promega Klenow Fragment, DNA Polymerase I
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Quantity:
150 U
Concentration:
5 U/μL
Description
- DNA-Dependent DNA Polymerase that Lacks the 5′→3′ Exonuclease Activity
- Use in a variety of molecular applications
- Heat-inactivate by heating at 75°C for 10 minutes
- Active in many Promega 1X restriction enzyme buffers
- Supplied at a concentration of 5u/μL
†DNA polymerase kit that includes 5μmoles each of dATP, dCTP, dGTP, and dTTP at 10mM in water.
Specifications
Specifications
Concentration | 5 U/μL |
Color | Colorless |
Components | 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT |
Purity | >80% |
pH | 7.2 |
For Use With (Application) | Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method |
Storage Buffer | 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol |
Content And Storage | -30°C to -10°C |
Source | Recombinant E. coli strain |
Quantity | 150 U |
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