Klotho, Rat anti-Mouse, Human, Clone: KL-234 , Millipore Sigma™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABN180725	MilliporeSigma MABN180725UL	25 μL
MABN1807100	MilliporeSigma MABN1807100UL	100 μL

Description

Anti-Klotho Antibody, clone KL-234, Cat. No. MABN1807, is a highly specific rat monoclonal antibody that targets Klotho and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Klotho (UniProt: Q9UEF7; also known as EC: 3.2.1.31) is encoded by the KL gene (Gene ID:9365) in human. Klotho is a homodimeric, highly N-glycosylated, single-pass type I membrane protein that is present in kidney, placenta, small intestine, and prostate tissue. In kidney it is predominantly present in cortical renal tubules. A soluble secreted form (isoform 2, ∽ 64 kDa) is present in serum and cerebrospinal fluid. The Klotho peptide generated by cleavage of the membrane-bound isoform (Isoform 1) is considered as an anti-aging circulating hormone that is reported to extend life span by inhibiting insulin/IGF1 signaling. It also acts as a cofactor for interaction of FGF23 with FGF R1, which negatively regulates the activity of 1-alpha-hydroxylase, the rate limiting enzyme in the synthesis of 1,25(OH)2D3. Klotho is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form that has an extracellular domain (aa 31-981), a short transmembrane domain (aa 982-1002), and a short cytoplasmic tail (aa 1003-1012). Deficiency of Klotho is reported to shorten life-span in animal models with a myriad of disorders associated with old age, such as arteriosclerosis, osteoporosis, and skin atrophy. Klotho deficient mice also display severe hyperphosphatemia and soft tissue calcification. On the contrary, over-expression of Klotho is shown to extend life-span in murine models by about 30% with side effects, such as mild insulin resistance and enhanced resistance to oxidative stress. Defects in KL gene has been linked to chronic renal failure complications.

Specifications

• Antigen: Klotho
• Dilution: Western Blotting Analysis: A 1:1,000 dilution from a represetative lot detected Klotho in 10μg of mouse kidney tissue lysates.Immunohistochemistry Analysis: A representative lot detected Klotho in Immunohistochemistry applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).Immunoprecipitation Analysis: A representative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).Western Blotting Analysis: A representative lot detected Klotho in Western Blotting applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).Immunocytochemistry Analysis: A representative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).
• Classification: Monoclonal
• Form: Ascites
• Regulatory Status: RUO
• Target Species: Mouse, Human
• Gene Alias: EC: 3.2.1.31;KL
• Gene Symbols: KL
• Isotype: IgG2a κ
• Purification Method: Unpurified
• Test Specificity: Clone KL-234 is a rat monoclonal antibody that specifically detects Klotho. It targets an epitope with in 107 amino acids from the KL1 domain from the N-terminal region.

• Clone: KL-234
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rat
• Research Discipline: Neuroscience
• Formulation: Rat monoclonal antibody as ascites with 0.1% sodium azide with 50% glycerol.
• Gene ID (Entrez): NP_004786
• Immunogen: GST-tagged recombinant fragment corresponding to 107 amino acids from the extracellular domain from the N-terminal region of human Klotho.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.