L1/ORF2 Antibody, Novus Biologicals™

Chicken Polyclonal Antibody

Supplier:  Novus Biologicals NBP177929

Catalog No. NBP177929

Description

L1/ORF2 Polyclonal specifically detects L1/ORF2 in Human, Chimpanzee samples. It is validated for Western Blot, ELISA.

Specifications

• Antigen: L1/ORF2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: EC=2.7.7.49, EC=3.1.21.-, Endonuclease, L1, LINE-1 retrotransposable element ORF2 protein, Long interspersed element-1, ORF2p, Retrovirus-related Pol polyprotein LINE-1, Reverse transcriptase
• Host Species: Chicken
• Molecular Weight of Antigen: 149.01 kDa
• Quantity: 0.1 mg
• Primary or Secondary: Primary
• Test Specificity: L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

• Applications: Western Blot, ELISA
• Concentration: 1 mg/ml
• Dilution: Western Blot 1:2000-1:10000, ELISA 1:10000
• Gene Accession No.: Q5BJF6
• Gene Symbols: ODF2
• Immunogen: This IgY fraction L1/ORF2 Antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein. (Uniprot: O00370)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 4957
• Target Species: Human, Chimpanzee
• Isotype: IgG

For Research Use Only