Lactate Dehydrogenase Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-0445	100 μg
50-199-0446	25 μL

Catalog No. 50-199-0445 Supplier Rockland Immunochemicals Supplier No. 20011730100

Description

Goat Polyclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-LACTATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

Specifications

• Antigen: Lactate Dehydrogenase
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: LDHA
• Gene Accession No.: P13491
• Gene Alias: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; l7R2; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase A; lactate dehydrogenase A4; lactate dehydrogenase A-like; lactate dehydrogenase M; lactate dehydrogenase-A; lactate dehydrogenase-M; Lactic Dehydrogenase; LDH; LDH muscle subunit; Ldh1; Ldh-1; ldha; LDH-A; ldha protein; ldha1; LDH-A4 muscle-specific isozyme; glycolytic enzyme; LDHM; LDH-M; L-lactate dehydrogenase; L-lactate dehydrogenase A chain; M-LDH; OTTHUMP00000165206; PIG19; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59
• Gene Symbols: LDHA
• Host Species: Goat
• Immunogen: This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100009107
• Target Species: Rabbit
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized