LAG-3 (CD223) Mouse anti-Human, APC-R700, Clone: T47-530, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 565775

Catalog No. BDB565775

Description

The T47-530 specifically recognizes the Lymphocyte Activation Gene 3 (LAG-3) protein which is also known as, Protein FDC, or CD223. LAG-3 is a ñ70 kDa type I transmembrane glycoprotein that belongs to the Ig superfamily and exhibits homology to CD4. LAG-3 is expressed on NK cells, regulatory T cells, and activated conventional T cells with higher expression found on CD8+ T cells compared with CD4+ T cells. LAG-3 is an activation induced cell surface molecule that like CD4, binds MHC class II molecules, but with much higher affinity. This may enable LAG-3 to act as a negative competitor of CD4 for MHC class II ligand binding. LAG-3 may associate with the TCR-CD3 complex to downregulate TCR signal transduction and T cell clonal expansion. In contrast, LAG-3-induced signaling may promote dendritic cell activation.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45nm filter). APC-R700 is a tandem fluorochrome that combines APC with R700, a proprietary organic dye. This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor™ 700. Due to similar excitation and emission properties, APC-R700 and Alexa Fluor™ 700 cannot be used simultaneously.

Specifications

• Antigen: LAG-3 (CD223)
• Clone: T47-530
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: Mouse IgG1 κ

• Classification: Monoclonal
• Conjugate: APC-R700
• Gene Alias: LAG3, CD223, FDC, Lymphocyte activation gene 3 protein, Protein FDC
• Immunogen: Human LAG-3 Recombinant Protein
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.