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Thermo Scientific™ LanthaScreen™ IGF-1R GripTite™ Cell Line

Catalog No. K1834
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Catalog No. K1834 Supplier Thermo Scientific™ Supplier No. K1834
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Description

The IGF-1R/PI3K/AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. IGF-1R is a receptor tyrosine kinase (RTK) that resides at the top of the pathway.

The IGF-1R/PI3K/AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. IGF-1R is a receptor tyrosine kinase (RTK) that resides at the top of the pathway. Upon growth factor binding extracellularly, this RTK undergoes auto-phosphorylation at multiple intracellular tyrosine residues (resulting in pathway activation).

The LanthaScreen™ IGF-1R GripTite™ (HEK 293 MSR) cellular assay utilizes a human cell line that constitutively expresses IGF-1R fusion proteins with yellow fluorescent protein (YFP). This kinase target was introduced using lipid transfection and these cells are a clonal population isolated by FACS, using YFP fluorescence as a sorting marker and Blasticidin to maintain cells under selection. Using this cell line, a homogenous immunoassay was developed with a time-resolved FRET (TR-FRET) readout in which the insulin-induced phosphorylation of multiple tyrosine residues on IGF-1R is detected in cell lysates using a generic terbium-labeled phosphospecific antibody (Tb-anti-pY20). This cell line has been validated with different stimuli/inhibitors and shows correct EC50/IC50 values. Moreover, this assay has been optimized for performance under variable experimental conditions (including cell plating density, agonist stimulation time, DMSO tolerance and assay development time) and displays excellent statistical data (Z' > 0.6) and good signal-to-background.

TRUSTED_SUSTAINABILITY

Specifications

Antagonists IGF-1R Inhibitor II
Assay Entry Cell-based terbium phospho-specific activity (stable)
Cell State Dividing Cells
Content And Storage LanthaScreen™ IGF-1R GripTite™ cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method Fluorescence
For Use With (Application) TR-FRET
Gene Symbol IFG-1R
Human Protein Class Membrane Proteins
Primary Agonist IGF-1
Product Line LanthaScreen
Quantity 1 Vial
Readout End Point
Reporter Gene GFP (EmGFP)
Target Entry IGF-1R
Type Cell Line
Cell Line 293 GripTite MSR
System Type LanthaScreen™
Druggable Target Kinases
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For Research Use Only. Not for use in diagnostic procedures.

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