Invitrogen™ LAT Monoclonal Antibody (LAT.10-17 (10-17)), eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-139-36	Invitrogen™ 14996782	100 μg

Description

Description: This LAT.10-17 (10-17) monoclonal antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated signal transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream signaling molecules such as Grb-2, PLCgamma, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, natural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen. Applications Reported: This LAT.10-17 (10-17) antibody has been reported for use in immunoblotting (WB) and immunoprecipitation (IP). Applications Tested: LAT.10-17 (10-17) was tested by western blot and immunoprecipitation on lysates prepared from human peripheral blood cells, Jurkat cells, and C57BL/6 splenocytes. This antibody can be used at 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%,...

LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.

Specifications

• Antigen: LAT
• Concentration: 0.5 mg/mL
• Applications: Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O43561
• Gene ID (Entrez): 27040
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: LAT.10-17 (10-17)
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: LAT
• Gene Alias: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; Lat; LAT1; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; linker for activation of T-cells; linker for activation of T-cells family member 1; linker protein; p36-38; pp36
• Gene Symbols: LAT
• Product Type: Antibody