Invitrogen™ iBlot™ 2 Gel Transfer Device

Performs Western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers

Supplier:  Invitrogen™ IB21001

Catalog No. IB21001

Description

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

• Content And Storage: Store at room temperature.
• For Use With (Equipment): iBlot™
• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: iBlot
• Throughput: 1 Midi or 2 Mini Gels
• Running Dimension: Horizontal

• For Use With (Application): Western Blot
• Gel Compatibility: Bolt™ Bis-Tris Plus Gel, E-PAGE™ Gel, Novex™ Midi Gel, NuPAGE™ Gel, Novex™ Mini Gel
• Mode of Transfer: Dry
• Running Time: ∼7 min.
• Type: Transfer Device

For Research Use Only. Not for use in diagnostic procedures.