Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Supplier:  Thermo Scientific™ K0373

Catalog No. FERK0373

Includes: Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dy

Description

Thermo Scientific™ Luminaris Color HiGreen Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for SYBR Green chemistry using standard cycling protocols.

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance
• Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity: single copy detection
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2X master mix

Specifications

• Concentration: 2X
• Detection Method: SYBR
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: Luminaris
• Quantity: 1250 reactions
• For Use With (Application): Gene Expression
• No. of Reactions: 1250 Reactions

• Content And Storage: Contains:
  • 10 x 1.25 mL Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 1250 x 20 μL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
  • 3 x 1.25 mL 40X Yellow Sample Buffer
  • 10 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
• Product Type: qPCR Master Mix
• Sufficient For: 250 Reactions
• Fidelity (vs. Taq): 2 X
• Sample Type: DNA, dsDNA

For Research Use Only. Not for use in diagnostic procedures.