Ly-49G2 Monoclonal Antibody (eBio4D11 (4D11)), FITC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-985-1	Invitrogen™ 11578182	100 μg

Description

Description: The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1). The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains, which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor. Ly-49G2 is an inhibitory receptor expressed on subsets of natural killer (NK) cells, NK-T cells (in all strains tested), and on a population of memory CD8+ T cells (in C57BL/6 mice). The 4D11 antibody has been reported to cross react with the Ly-49AB6, Ly-49ABALB, and Ly-49T129/J inhibitory receptors and Ly-49LCBA/J activatingreceptors. The proportion of NK-T cells expressing Ly-49A and Ly-49G2 is higher in the thymus than in liver, and there is evidence that down-regulation of Ly-49 receptor expression is necessary for normal NK-T-cell development to occur. Applications Reported: This eBio4D11 (4D11) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio4D11 (4D11) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.

Specifications

• Antigen: Ly-49G2
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q60654
• Gene Symbols: Klra7
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Klra7

• Clone: eBio4D11 (4D11)
• Applications: Flow Cytometry
• Conjugate: FITC
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 16638
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.