Invitrogen™ Ly-6K Monoclonal Antibody (B33fc8k), PE, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2357	Invitrogen™ 12592982	100 μg

Description

Description: This B33fc8k monoclonal antibody reacts with mouse Ly-6k, a member of the Ly6/uPAR superfamily. Ly-6k is a GPI linked protein predominantly found on the cell surface. One study suggests expression may also be non-membranous. Expression is found on B cells and reported to be on plasma cells as well as cells in the testis. Using the B33fc8k monoclonal antibody, expression is on a B lymphocyte cell subset in the spleen that correlates to transitional cells (CD23-IgM-). In humans Ly-6k has been found associated with carcinomas of the head and testis. Applications Reported: This B33fc8k antibody has been reported for use in flow cytometric analysis. Applications Tested: This B33fc8k antibody has been tested by flow cytometric analyis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida. May play a role in cell growth (PubMed:18089789). [UniProt].

Specifications

• Antigen: Ly-6K
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9CWP4
• Gene ID (Entrez): 76486
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: B33fc8k
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Mouse
• Gene: LY6K
• Gene Alias: 2410015A16Rik; 3110035B01Rik; cancer/testis antigen 97; CO16; CT97; FLJ35226; HSJ001348; Ly6k; ly-6K; lymphocyte antigen 6 complex, locus K; lymphocyte antigen 6 complex, locus K-like; lymphocyte antigen 6K; mLy-6K; up-regulated in lung cancer 10; URLC10
• Gene Symbols: LY6K
• Product Type: Antibody