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M-CSF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
$250.00 - $2175.00
Specifications
Antigen | M-CSF |
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Concentration | 0.1-1.0 mg/mL |
Applications | ELISA, Western Blot |
Classification | Polyclonal |
Conjugate | Biotin |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
500P44BT25
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Invitrogen™
500P44BT25UG |
25 μg |
Each for $250.00
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500P44BT50
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Invitrogen™
500P44BT50UG |
50 μg |
Each for $319.00
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50-271-8485
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Invitrogen™
500P44BT500UG |
500 μg |
Each for $2,175.00
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Description
AA Sequence of recombinant protein: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human M-CSF. Anti-Human M-CSF-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human M-CSF (500-P44) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P44BT-1MG will be provided as 2 x 500 μg
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.Specifications
M-CSF | |
ELISA, Western Blot | |
Biotin | |
Rabbit | |
Human | |
P09603 | |
1435 | |
E.coli-derived Recombinant Human M-CSF. | |
Antigen Affinity Chromatography | |
Csf1 |
0.1-1.0 mg/mL | |
Polyclonal | |
Lyophilized | |
RUO | |
PBS with no preservative | |
C87615; colony stimulating factor 1; colony stimulating factor 1 (macrophage); colony-stimulating factor-1 splice variant; CSF1; CSF-1; Csfm; H-MCSF; lanimostim; macrophage colony stimulating factor 1; macrophage colony stimulating factor alpha; macrophage colony stimulating factor beta; macrophage colony-stimulating factor; macrophage colony-stimulating factor 1; macrophage colony-stimulating factor beta; macrophage-colony stimulating factor alpha; MCSF; MCSF alpha; MCSFBETA; MGC31930; M-MCSF; op; osteopetrosis; Processed macrophage colony-stimulating factor 1 | |
Csf1 | |
Primary | |
-20°C |
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