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MARK2, p Thr595 Antibody, Novus Biologicals™
SDP

Catalog No. NBP178028 Shop All R&D Systems Products
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NBP178028 0.1 mg
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Catalog No. NBP178028 Supplier Novus Biologicals Supplier No. NBP178028
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Rabbit Polyclonal Antibody

MARK2 Polyclonal specifically detects MARK2 in Human samples. It is validated for Western Blot, ELISA, Immunocytochemistry/Immunofluorescence.

Specifications

Antigen MARK2 (p Thr595)
Applications Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Classification Polyclonal
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1:250-1:1000, ELISA 1:1000-1:3000, Immunocytochemistry/Immunofluorescence 1:10-1:500
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Accession No. Q7KZI7
Gene Alias EC 2.7.11, EC 2.7.11.1, ELKL motif kinase, ELKL motif kinase 1, EMK-1, EMK1PAR-1, MAP/microtubule affinity-regulating kinase 2MGC99619, PAR1 homolog, Par1b, Ser/Thr protein kinase PAR-1B, serine/threonine protein kinase EMK, serine/threonine-protein kinase MARK2
Gene Symbols MARK2
Host Species Rabbit
Immunogen This affinity purified MARK2 [p Thr595] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595. (Uniprot: Q7KZI7)
Molecular Weight of Antigen 87.91 kDa
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Phospho Specific, Protein Kinase
Primary or Secondary Primary
Gene ID (Entrez) 2011
Test Specificity MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.
Target Species Human, Mouse, Rat, Bovine, Canine
Content And Storage Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype IgG
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