Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit

Description

Includes

First Strand Enzyme Mix, 4X First Strand Reaction Mix, Second Strand Enzyme Mix, 5X Second Strand Reaction Mix, 0.5M EDTA ( pH 8.0), RNase I (10 u/μL), Control RNA (0.5μg/μL), Oligo(dT)₁₈ Primer, 100μM, 0.5μg/μL, Random Hexamer Primer, 100μM, 0.2μg/μL,Water, nuclease-free, Detailed Protocol

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.

Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains pre-mixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Maxima H Minus Double-Stranded cDNA Synthesis Kit include:

• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in less than two hours
• Convenient—pre-mixed components
• Complete—includes all primers, controls and residual RNA removal reagents

Applications

• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction
  
  

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

• Format: 10 reactions
• Optimal Reaction Temperature: 50°-55° C
• Product Line: Maxima
• Product Type: cDNA Synthesis Kit
• Quantity: 10 Reactions
• Reverse Transcriptase: Maxima H Minus
• For Use With (Application): Cloning
• Final Product Type: Double Stranded cDNA
• No. of Reactions: 10 Reactions
• Reaction Format: Separate Components
• Sample Type: RNA

For Research Use Only. Not for use in diagnostic procedures.