Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit PROMO

Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.

Supplier:  Thermo Scientific™ K1651

Catalog No. FERK1651PM

Description

This kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

For reverse transcription the kit uses Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitroevolution of MMLV RT.

The Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand-specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in two minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved, and dsDNase treated RNA can be directly added to reverse transcription.

Features of the Maxima H Minus First Strand cDNA Synthesis Kit include:

• Increased reaction temperatures–the first strand of cDNA can be synthesized within the 42-65°C temperature range
• High yields of full-length first strand cDNA–with RNA templates up to 20 kb
• Flexible priming–oligo(dT)₁₈, random hexamer or gene-specific primers
• Integrated genomic DNA removal step with dsDNase kit

Applications

• First Strand cDNA synthesis for RT-PCR
• Construction of cDNA libraries
• Generation of probes for hybridization
• Antisense RNA synthesis

Contents

• Maxima H Minus Enzyme Mix
• Oligo(dT)₁₈ and Random hexamer primers
• 5X RT Buffer
• dNTP Mix
• Nuclease-free water

First Strand Synthesis Kit with dsDNase also contains

• dsDNase and 10X dsDNase Buffer

Additional information about reaction components

• Maxima H Minus Enzyme Mix contains Maxima H Minus Reverse Transcriptase and RiboLock RNase Inhibitor. RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B, and C at temperatures up to 55°C.
• Oligo(dT)₁₈ and random hexamer primers are supplied with the kit. Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population. The oligo(dT)18 primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A) tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence.
• 10 mM dNTP Mix is a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.
• Nuclease-free water is provided for reaction set-up and dilution of sample DNA. The absence of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.

Specifications

• Content And Storage: Maxima H Minus Enzyme Mix, Oligo(dT)₁₈ and Random hexamer primers, 5X RT Buffer, dNTP Mix, Nuclease-free water, Store at -20°C.
• GC-Rich PCR Performance: High
• Technique: Reverse Transcription
• Reverse Transcriptase: Maxima H Minus
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reaction Format: Separate components
• Size (Final Product): Up to 20 kb
• Includes: Kit only

• Format: Kit
• Reaction Speed: 30 min.
• Optimal Reaction Temperature: 50°C to 55°C
• Ribonuclease H Activity: Reduced
• For Use With (Application): Real Time PCR (qPCR), RT-PCR
• No. of Reactions: 20 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.