Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit

Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.

$193.00 - $831.00

Specifications

Format Kit
GC-Rich PCR Performance High
Reaction Speed 30 min.
Technique Reverse Transcription
Optimal Reaction Temperature 50°C to 55°C
View More Specs

Products 4
Catalog Number Mfr. No. Includes No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. Includes No. of Reactions Price Quantity & Availability  
FERK1651
View Documents
Thermo Scientific™
K1651
Kit only 20 Reactions
Each for $193.00
Add to Cart
 
FERK1652
View Documents
Thermo Scientific™
K1652
Kit only 100 Reactions
Each for $751.00
Add to Cart
 
FERK1681
View Documents
Thermo Scientific™
K1681
Kit with dsDNase 20 Reactions
Each for $210.00
Add to Cart
 
FERK1682
View Documents
Thermo Scientific™
K1682
Kit with dsDNase 100 Reactions
Each for $831.00
Add to Cart
 
Description

Description

Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of MMLV RT. The enzyme features the highest thermostability among the derivatives of MMLV RT and lacks RNase H activity.

This kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

For reverse transcription the kit uses Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of MMLV RT.

The Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand-specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in two minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved, and dsDNase treated RNA can be directly added to reverse transcription.

Features of the Maxima H Minus First Strand cDNA Synthesis Kit include:
• Increased reaction temperatures—the first strand of cDNA can be synthesized within the 42–65°C temperature range
• High yields of full-length first strand cDNA—with RNA templates up to 20 kb
• Flexible priming—oligo(dT)18, random hexamer or gene-specific primers
• Integrated genomic DNA removal step with dsDNase kit

Applications
• First Strand cDNA synthesis for RT-PCR
• Construction of cDNA libraries
• Generation of probes for hybridization
• Antisense RNA synthesis

Contents
• Maxima H Minus Enzyme Mix
• Oligo(dT)18 and Random hexamer primers
• 5X RT Buffer
• dNTP Mix
• Nuclease-free water

First Strand Synthesis Kit with dsDNase also contains
• dsDNase and 10X dsDNase Buffer

Additional information about reaction components
• Maxima H Minus Enzyme Mix contains Maxima H Minus Reverse Transcriptase and RiboLock RNase Inhibitor. RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B, and C at temperatures up to 55°C.
• Oligo(dT)18 and random hexamer primers are supplied with the kit. Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population. The oligo(dT)18 primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A) tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence.
• 10 mM dNTP Mix is a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.
• Nuclease-free water is provided for reaction set-up and dilution of sample DNA. The absence of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests.

Specifications

Specifications

Kit
30 min.
50°C to 55°C
Reduced
First-Strand cDNA
Reverse Transcription
RNA
High
Reverse Transcription
Maxima H Minus
Dry Ice
Separate components
Up to 20 kb
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit