Maxima H Minus First Strand cDNA Synthesis Kit† is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.
The new Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.
- Integrated genomic DNA removal step
- Increased reaction temperatures -the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
- High yields of full-length first strand cDNA -with RNA templates up to 20 kb
- Flexible priming -oligo(dT)18, random hexamer, or gene-specific primers
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|Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)|
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