Invitrogen™ MCP-5 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P61BT500UG

Catalog No. 50-271-8467

Description

AA Sequence of recombinant protein: GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MCP-5 (CCL12). Anti-Murine MCP-5 (CCL12)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect mMCP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-5 (500-P61) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P61BT-1MG will be provided as 2 x 500 μg

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

Specifications

• Antigen: MCP-5
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Ccl12
• Gene Alias: C-C motif chemokine 12; Ccl12; chemokine (C-C motif) ligand 12; MCP-1-related chemokine; Mcp5; MCP-5; Monocyte chemoattractant protein 5; monocyte chemotactic protein 5; Scya12; small inducible cytokine A12; small-inducible cytokine A12
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20293
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q62401
• Gene Symbols: Ccl12
• Immunogen: E.coli-derived Recombinant Murine MCP-5 (CCL12).
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG