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MDM2/HDM2 Antibody (SMP14), Alexa Fluor™ 594, Novus Biologicals™
SDP

Catalog No. N102736A594 Shop All R&D Systems Products
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Catalog No. N102736A594 Supplier Novus Biologicals Supplier No. NB1002736AF594
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Description

Mouse Monoclonal Antibody

MDM2/HDM2 Monoclonal specifically detects MDM2/HDM2 in Human, Mouse, Rat samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

Antigen MDM2/HDM2
Applications Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone SMP14
Conjugate Alexa Fluor 594
Dilution Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias ACTFS, HDM2, HDMX, MDM2 oncogene, E3 ubiquitin protein ligase
Gene Symbols MDM2
Host Species Mouse
Immunogen Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProtNo. Q00987]
Purification Method Protein G purified
Quantity 0.1 mL
Research Discipline Cancer, Cell Cycle and Replication, Cellular Markers, Hypoxia, Neuroscience, p53 Pathway, Phospho Specific, Tumor Suppressors
Primary or Secondary Primary
Gene ID (Entrez) 4193.0
Test Specificity The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Target Species Human, Mouse, Rat
Content And Storage Store at 4°C in the dark.
Isotype IgG1 κ
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