Melan-A/MART-1 Antibody (M2-7C10), DyLight 650, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP233148C

Catalog No. NBP233148C

Description

Melan-A/MART-1 Monoclonal specifically detects Melan-A/MART-1 in Human, Equine, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Melan-A/MART-1
• Classification: Monoclonal
• Conjugate: DyLight 650
• Gene Alias: Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG2b λ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: M2-7C10
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Gene Symbols: MLANA
• Immunogen: Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)
• Quantity: 0.1 mL
• Research Discipline: Cytoskeleton Markers, Immunology
• Gene ID (Entrez): 2315
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Form: Purified

For Research Use Only