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Melan-A/MART-1 Mouse, Clone: M2-7C10 + M2-9E3, Novus Biologicals™
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Catalog No. NBP234245F Shop All R&D Systems Products
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NBP234245F 0.02 mg
NBP234245A 0.1 mg
NBP234245B 0.2 mg
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Catalog No. NBP234245F Supplier Novus Biologicals Supplier No. NBP2342450.02MG
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Description

Mouse Monoclonal Antibody

Melan-A/MART-1 Monoclonal specifically detects Melan-A/MART-1 in Human, Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

Antigen Melan-A/MART-1
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone M2-7C10 + M2-9E3
Conjugate Unconjugated
Dilution Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Formulation PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Accession No. Q16655
Gene Alias Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A
Gene Symbols MLANA
Host Species Mouse
Immunogen Recombinant hMART-1 protein (M2-7C10; M2-9E3)
Molecular Weight of Antigen 21 kDa
Purification Method Protein A purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cytoskeleton Markers, Immunology
Primary or Secondary Primary
Gene ID (Entrez) 2315
Test Specificity This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Target Species Human, Mouse, Rat
Content And Storage Store at 4C.
Form Purified
Isotype IgG
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