MFG-E8 Antibody (EDM45) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247813A	Novus Biologicals NBP2478130.1MG	0.1 mg
NBP247813B	Novus Biologicals NBP2478130.2MG	0.2 mg

Description

MFG-E8 Monoclonal specifically detects MFG-E8 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: MFG-E8
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 4240
• Immunogen: Delipidated human milk fat globule membrane preparation
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 45 kDa

• Clone: EDM45
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: BA46, Breast epithelial antigen BA46, EDIL1, hP47, HsT19888, lactadherin, medin, MFG1, MFG-E8, MFGM, Milk fat globule-EGF factor 8, milk fat globule-EGF factor 8 protein, O-acetyl disialoganglioside synthase, OAcGD3S, SED1, SPAG10, sperm associated antigen 10, sperm surface protein hP47
• Gene Symbols: MFGE8
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.