MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-599-881	Invitrogen 14599881	50 μg
50-134-42	Invitrogen 14599882	100 μg
50-134-43	Invitrogen 14599885	500 μg

Description

Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p. Applications Reported: 34-1-2S has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen mouse tissue sections, and complement-dependent cytotoxicity. Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class I (H-2Kd/H-2Dd)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2-D1; H2-DC1-beta transplantation antigen protein; H2DD; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2K1; H2-K1; H2KD; histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein
• Gene Symbols: H2-D1, H2-K1
• Primary or Secondary: Primary
• Content And Storage: 4° C

• Clone: 34-1-2S
• Applications: Flow Cytometry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Mouse
• Gene Accession No.: P01895, P01896, P01897, P01899, P01900, P01901, P03991, P04223, P14426, P14427, P14428
• Gene ID (Entrez): 14964, 14972
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.