MHC Class II Antibody (M5/114) - Azide and BSA Free, Novus Biologicals™

Rat Monoclonal Antibody has been used in 3 publications

Supplier:  Novus Biologicals NBP221789

Catalog No. NBP221789

Description

MHC Class II Monoclonal specifically detects MHC Class II in Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Functional, CyTOF-ready.

Specifications

• Antigen: MHC Class II
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot 1:500-1:2000, Flow Cytometry 2 μg/mL, Immunohistochemistry 5 to 10 μg/mL, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:300, Immunohistochemistry-Frozen 1:50-1:300, Functional, CyTOF-ready
• Gene Symbols: HLA-DMA
• Immunogen: Activated C57BL/6 mouse spleen cells
• Quantity: 0.1 mg
• Research Discipline: Adaptive Immunity, Asthma, Cell Biology, Diabetes Research, Immunology, Major Histocompatibility Complexes
• Gene ID (Entrez): 3108
• Target Species: Mouse, Rat
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: M5/114
• Conjugate: Unconjugated
• Gene Alias: class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
• Host Species: Rat
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
• Content And Storage: Store at 4C. Do not freeze.
• Isotype: IgG2b

For Research Use Only