MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free, Novus Biologicals™

Rat Monoclonal Antibody has been used in 11 publications

Supplier:  Novus Biologicals NBP1433120.05MG

Catalog No. NBP14331205

Description

MHC class II (I-A/I-E) Monoclonal specifically detects MHC class II (I-A/I-E) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: MHC Class II
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry < 0.125 ug/10^6 cells, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Gene Alias: class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
• Content And Storage: Store at 4C. Do not freeze.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: M5/114.15.2
• Conjugate: Unconjugated
• Formulation: Aqueous, may contain carrier protein/stabilizer. with 0.09% Sodium Azide
• Gene Symbols: HLA-DMA
• Immunogen: The immunogen for this antibody was MHC Class II.
• Quantity: 0.05 mg
• Research Discipline: Adaptive Immunity, Asthma, Cell Biology, Diabetes Research, Immunology, Major Histocompatibility Complexes
• Gene ID (Entrez): 3108
• Target Species: Mouse
• Isotype: IgG2b κ

For Research Use Only