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Invitrogen™ MIF Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA595222
Description
Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human Jurkat whole cell, human K562 whole cell, human Hela whole cell, human HepG2 whole cell. IHC: human liver cancer tissue, human lung cancer tissue. ICC/IF: Hela cell. Flow: Jurkat cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
Specifications
MIF | |
Polyclonal | |
Unconjugated | |
Mif | |
cytokine; delayed early response protein 6; DER6; GIF; GLIF; glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MGC107654; Mif; MMIF; phenylpyruvate tautomerase | |
Rabbit | |
Affinity Chromatography | |
RUO | |
4282 | |
-20°C | |
Lyophilized |
Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry | |
500 μg/mL | |
PBS with 4mg trehalose and no preservative | |
P14174 | |
Mif | |
E.coli-derived human MIF recombinant protein (Position: P2-A115). | |
100 μg | |
Primary | |
Human | |
Antibody | |
IgG |
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