MLH1 Mouse anti-Human, Clone: MLH1/1324, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2476560.1MG

Catalog No. NBP247656A

Description

MLH1 Monoclonal specifically detects MLH1 in Human samples. It is validated for ELISA.

Specifications

• Antigen: MLH1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: MLH1
• Immunogen: Recombinant human MLH1 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes a protein of 83kDa, identified as MLH1. Its epitope maps in aa 380-410. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.
• Content And Storage: Store at 4C.
• Isotype: IgG2b

• Applications: Western Blot, SDS-Page
• Clone: MLH1/1324
• Dilution: Western Blot 0.5-1.0ug/ml, SDS-Page
• Gene Alias: COCA2FCC2, DNA mismatch repair protein Mlh1, hMLH1, HNPCC, HNPCC2MGC5172, mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2), mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), MutL protein homolog 1
• Host Species: Mouse
• Molecular Weight of Antigen: 85 kDa
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, Checkpoint signaling, DNA Double Strand Break Repair, DNA Repair, Mismatch Repair, Tumor Suppressors
• Gene ID (Entrez): 4292
• Target Species: Human
• Form: Purified