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Invitrogen™ CellLight™ Histone 2B-GFP, BacMam 2.0
Description
This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to histone 2B. You can observe histone 2B-GFP behavior in live cells using fluorescent imaging with almost no cytotoxicity.
Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.
BacMam Technology
CellLight Histone 2B-GFP, BacMam 2.0, is a fusion construct of histone 2B and emGFP, providing accurate and specific targeting to cellular histone 2B-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
- Fast and convenient: simply add CellLight reagent to cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
- Highly efficient: up to 90% transduction of wide range of mammalian cell lines, including primary cells, stem cells, and neurons
- Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying dose
- Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
- Flow Cytometer Laser Lines: 488
Cell Analysis, Cell Structure, Cellular Imaging, Immunocytochemistry (ICC), Immunofluorescence (IF), Nucleus, Nucleoli & Nuclear Envelope
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Color | Green |
| Content And Storage | Store at 2°C to 6°C, protected from light. Do Not Freeze. |
| Excitation Wavelength Range | 488⁄510 |
| Dye Type | GFP (EmGFP) |
| For Use With (Equipment) | Confocal Microscope, Fluorescence Microscope |
| Quantity | 1 mL |
| Detection Method | Fluorescence |
| Emission | Visible |
| Form | Liquid |
| Product Line | CellLight™ |
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For Research Use Only. Not for use in diagnostic procedures.
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