Invitrogen™ Fura Red™, AM, cell permeant

Catalog No.	Quantity
F3021	10 x 50 μg
F3020	500 μg

Catalog No. F3021 Supplier Invitrogen™ Supplier No. F3021

Description

Visible light-excitable fura-2 analog that offers particular possibilities for ratiometric measurement of Ca²⁺ in single cells

Fura Red, AM is a visible light—excitable fura-2 analog that offers unique possibilities for ratiometric measurement of Ca²⁺ in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading and is also available in 1 mg amounts (F-3020).

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): Calcium Indicator
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Product Type: Fura Red AM
• Dye Type: Fura Red
• Product Line: Fura Red
• Quantity: 10 x 50 μg
• Shipping Condition: Room Temperature
• Excitation Wavelength: 488 nm
• Emission Wavelength: 598 nm

Frequently Asked Questions (FAQs)

When using Calcium Crimson, AM, as an indicator of intracellular calcium flux, I am not getting a good degree of change. The cells also have GFP. What can I do?

This is a known drawback of Calcium Crimson, AM (as well as Calcium Orange, AM and Fura Red, AM, which are also in the same emission range). You can try increasing the concentration and washing out of any unlabeled dye from the media, to try to get better signal-to-background. If that fails, we recommend using Rhod-3 AM instead, which has a much better change in signal in that wavelength.

Where can I find the manual for the Fura Red, AM, cell permeant (Cat. No. F3020, F3021)?

Unfortunately, we do not have a user manual for the Fura Red, AM, cell permeant (Cat. No. F3021). You can reference section 19.3 of the Molecular Probes handbook for recommendations for use.
There is also a large number of citations & references provided on the product page.

Basically, you can dissolve the product in good quality anhydrous DMSO to prepare a stock solution of 1-10 mM and use a final solution of about 1-10 µM in a physiological buffer. You can then incubate for 15 to 60 minutes, wash and then leave the cells for about 30 minutes to allow the AM group to hydrolyse before starting the assay. You can add Pluronic F-127 (e.g., Cat. No. P6866) to facilitate uptake. Store the DMSO stock solution at -20 degrees C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.