Invitrogen™ SBFI, AM, cell permeant - Special Packaging

Catalog No.	Quantity
S1264	20 x 50 μg

Catalog No. S1264 Supplier Invitrogen™ Supplier No. S1264

Description

Sodium-sensitive molecule used to estimate Na⁺ gradients in isolated mitochondria, to measure intracellular NA⁺ levels, or to measure Na⁺ efflux in cells

• In combination with other fluorescent indicators used to correlate changes in intracellular Na⁺ with Ca²⁺ and Mg²⁺ concentrations, intracellular pH, and membrane potential
• Although selectivity of SBFI for Na⁺ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na⁺ in presence of other monovalent cations
• Spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-
• Label (Ex/Em): SBFI (approximately 340,380/500nm)
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding dissolved indicator to medium containing cells
• Selectivity Considerations and Cell Loading Strategies
• Dissociation constant (K_d) of SBFI for Na⁺ is 3.8mm in absence of K⁺, and 11.3 mm in solutions with combined Na⁺ and K⁺ concentration of 135 mm (which approximates physiological ionic strength)
• SBFI is approximately 18-fold more selective for Na⁺ than for K⁺

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Sodium and Potassium Detection, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): Cell Viability and Proliferation
• For Use With (Equipment): Fluorescence Microscope
• Product Type: SBFI AM
• Dye Type: Sodium Indicator
• Quantity: 20 x 50 μg
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.