Invitrogen™ SBFI, AM, cell permeant - Special Packaging

Description

Sodium-sensitive molecule used to estimate Na⁺ gradients in isolated mitochondria, to measure intracellular NA⁺ levels, or to measure Na⁺ efflux in cells

• In combination with other fluorescent indicators used to correlate changes in intracellular Na⁺ with Ca²⁺ and Mg²⁺ concentrations, intracellular pH, and membrane potential
• Although selectivity of SBFI for Na⁺ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na⁺ in presence of other monovalent cations
• Spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-
• Label (Ex/Em): SBFI (approximately 340,380/500nm)
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding dissolved indicator to medium containing cells
• Selectivity Considerations and Cell Loading Strategies
• Dissociation constant (K_d) of SBFI for Na⁺ is 3.8mm in absence of K⁺, and 11.3mm in solutions with combined Na⁺ and K⁺ concentration of 135mm (which approximates physiological ionic strength)
• SBFI is approximately 18-fold more selective for Na⁺ than for K⁺

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Sodium and Potassium Detection, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): Cell Viability and Proliferation
• For Use With (Equipment): Fluorescence Microscope
• Product Type: SBFI AM
• Dye Type: Sodium Indicator
• Quantity: 20 x 50 μg
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.