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Mouse anti-alpha-Fetoprotein/AFP, Clone: C3, Azide Free, Novus Biologicals™
SDP

Catalog No. NBP23457702 Shop All R&D Systems Products
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0.2 mg
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NBP23457702 0.2 mg
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Catalog No. NBP23457702 Supplier Novus Biologicals Supplier No. NBP2345770.2 MG

Mouse Monoclonal Antibody

alpha-Fetoprotein/AFP Monoclonal antibody specifically detects alpha-Fetoprotein/AFP in Human, Porcine, Canine, Primate, Bovine (Negative), Canine (Negative), Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready, Knockout Validated.

Specifications

Antigen alpha-Fetoprotein/AFP
Applications Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone C3
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
Formulation 10mM PBS - BSA free with No Preservative
Gene Accession No. P02771
Gene Alias Alpha-1-fetoprotein, Alpha-fetoglobulin, alpha-fetoprotein, FETA, HP, HPAFP
Gene Symbols AFP
Host Species Mouse
Immunogen Alpha fetoprotein (AFP) purified from serum of a hepatoma patient
Molecular Weight of Antigen 70 kDa
Purification Method Protein A purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Cancer, GPCR, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 174
Test Specificity It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Reconstitution Protein A purified
Target Species Human, Pig, Canine, Primate, Bovine (Negative), Canine (Negative), Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG2a κ
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