CD25 Mouse anti-Human, APC-R700, Clone: 2A3, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB565107	BD Biosciences 565107	25 Tests
BDB565106	BD Biosciences 565106	100 Tests

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL -2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).

Specifications

• Antigen: CD25
• Applications: Flow Cytometry
• Conjugate: APC-R700
• Regulatory Status: RUO
• Gene ID (Entrez): 100
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55

• Clone: 2A3
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Immunogen: Human Phytohemagglutinin-activated T Cells
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.