MilliporeSigma™ Mouse anti-CD8, Clone: SK1, PE,

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABF1676	MilliporeSigma™ MABF1676	25 Tests
MABF1677	MilliporeSigma™ MABF1677	100 Tests
MABF1678	MilliporeSigma™ MABF1678	500 Tests

Description

Specifically detects CD8 clone: SK1 in Human samples, and it is validated for Flow Cytometry

The SK1 antibody is specific for the 32-34kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The SK1 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, RPA-T8, the SK1 antibody will not block binding of RPA-T8 to CD8a.

Specifications

• Antigen: CD8
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Mouse
• Research Discipline: Inflammation and Immunology
• Formulation: Purified Mouse monoclonal containing 10mM NaH₂PO₄, 150mM NaCl, 0.09% NaN₃, 0.1% gelatin, pH 7.2.
• Gene Symbols: CD8A; MAL
• Primary or Secondary: Primary
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt. Note: It is recommended to store the product undiluted at 2°C to 8°C and protected from prolonged exposure to light. Do not freeze.

• Clone: SK1
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P01732
• Immunogen: Immunogen information for this antibody is proprietary information.
• Purification Method: Purified by size-exclusion chromatography

For Research Use Only