Mouse anti-Cd8a, Clone: C8/1035, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24532701	Novus Biologicals NBP2453270.1MG	0.1 mg
NBP24532702	Novus Biologicals NBP2453270.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD8 Monoclonal specifically detects CD8 in Human samples. It is validated for ELISA.

Specifications

• Antigen: CD8 alpha
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Innate Immunity
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 925
• Immunogen: Recombinant full-length human CD8a protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 32 kDa

• Clone: C8/1035
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: CD8, CD8 antigen, alpha polypeptide (p32), CD8a antigen, CD8a molecule, Leu2, Leu2 T-lymphocyte antigen, MAL, OKT8 T-cell antigen, p32, RPA-T8, RPA-T8 antibody flow, RPA-T8 CD8, RPA-T8 Clone, RPA-T8 Flow, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
• Gene Symbols: CD8A
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

For Research Use Only