Mouse anti-CDC2/CDK1, Clone: POH-1, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2347110.2 MG

Catalog No. NBP23471102

Description

CDC2/CDK1 Monoclonal antibody specifically detects CDC2/CDK1 in Human, Bovine, Mustelid, Primate, Drosophila (Negative), Mouse (Negative), Rat (Negative), Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: CDC2/CDK1
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1-2ug/ml, CyTOF-ready
• Gene Accession No.: P06493
• Gene Symbols: CDK1
• Immunogen: Purified recombinant human p34cdc2 fusion protein
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1/ p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1/ p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
• Target Species: Human, Bovine, Mustelid, Primate, Drosophila (Negative), Mouse (Negative), Rat (Negative), Xenopus (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: POH-1; same as cdc2.1
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: CDC28A, CDC2MGC111195, cell cycle controller CDC2, Cell division control protein 2 homolog, Cell division protein kinase 1, cyclin-dependent kinase 1, DKFZp686L20222, EC 2.7.11.22, EC 2.7.11.23, G1 to S and G2 to M, p34 protein kinase, P34CDC2
• Host Species: Mouse
• Molecular Weight of Antigen: 34 kDa
• Quantity: 0.2 mg
• Research Discipline: Breast Cancer, Cancer, Cell Cycle and Replication, Core ESC Like Genes, Mitotic Regulators, Phospho Specific, Stem Cell Markers
• Gene ID (Entrez): 983
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a κ

For Research Use Only