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Mouse anti-CEA, Clone: C66/261, Azide Free, Novus Biologicals™
SDP

Catalog No. NBP23458602 Shop All R&D Systems Products
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NBP23458602 0.2 mg
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Catalog No. NBP23458602 Supplier Novus Biologicals Supplier No. NBP2345860.2 MG

Description

Mouse Monoclonal Antibody

CEACAM5/CD66e Monoclonal antibody specifically detects CEACAM5/CD66e in Human, Primate samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

Antigen CEACAM5/CD66e
Applications Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone C66/261
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 1-2ug/ml, CyTOF-ready
Formulation 10mM PBS - BSA free with No Preservative
Gene Accession No. P06731
Gene Alias Carcinoembryonic antigen, carcinoembryonic antigen-related cell adhesion molecule 5, CD66e antigen, CEACD66e, DKFZp781M2392, Meconium antigen 100
Gene Symbols CEACAM5
Host Species Mouse
Immunogen Recombinant full-length human CEA protein
Purification Method Protein A purified
Quantity 0.2 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 1048
Test Specificity This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Reconstitution Protein A purified
Target Species Human, Primate
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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