Cytokeratin 8/18 Antibody (C-51), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Catalog Number	Mfr. No.	Quantity
NBP24492900	Novus Biologicals NBP2449290.02MG	0.02 mg
NBP24492901	Novus Biologicals NBP2449290.1MG	0.1 mg
NBP24492902	Novus Biologicals NBP2449290.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 8/18 Monoclonal specifically detects Cytokeratin 8/18 in Human, Rat, Porcine, Bovine, Ferret (Negative), Monkey, Sheep, Chicken (Negative), Donkey (Negative), Mouse (Negative), Rabbit (Negative), Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Cytokeratin 8/18
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cytoskeleton Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 3856
• Immunogen: Cytoskeleton preparation from HeLa cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: C-51
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Rat, Pig, Ferret (Negative), Monkey, Primate, Sheep, Chicken (Negative), Donkey (Negative), Mouse (Negative)
• Gene Alias: CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
• Gene Symbols: KRT8
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

For Research Use Only