Mouse anti-FoxP3, Clone: FXP3/197, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2346200.2 MG

Catalog No. NBP23462002

Description

FoxP3 Monoclonal antibody specifically detects FoxP3 in Human, Mouse, Primate samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: FoxP3
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Gene Accession No.: Q9BZS1
• Gene Symbols: FOXP3
• Immunogen: Full-length human FOXP3 protein
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.
• Target Species: Human, Mouse, Primate
• Form: Purified

• Applications: Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)
• Clone: FXP3/197
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: AIID, AIIDMGC141961, DIETER, forkhead box P3, Forkhead Box Protein P3, FOXP3delta7, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked, IPEX, JM2, MGC141961, MGC141963, PIDX, PIDXMGC141963, Scurfin, XPID, XPIDpolyendocrinopathy, enteropathy, X-linked
• Host Species: Mouse
• Molecular Weight of Antigen: 51 kDa
• Quantity: 0.2 mg
• Research Discipline: Adaptive Immunity, Cell Biology, Chromatin Research, Immunology, Transcription Factors and Regulators
• Gene ID (Entrez): 50943
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only