Mouse anti-FoxP3, Clone: FXP3/197, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP23462002	Novus Biologicals NBP2346200.2 MG	0.2 mg

Description

FoxP3 Monoclonal antibody specifically detects FoxP3 in Human, Mouse, Primate samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: FoxP3
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cell Biology, Chromatin Research, Immunology, Transcription Factors and Regulators
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: AIID, AIIDMGC141961, DIETER, forkhead box P3, Forkhead Box Protein P3, FOXP3delta7, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked, IPEX, JM2, MGC141961, MGC141963, PIDX, PIDXMGC141963, Scurfin, XPID, XPIDpolyendocrinopathy, enteropathy, X-linked
• Gene Symbols: FOXP3
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 51 kDa

• Clone: FXP3/197
• Dilution: Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Primate
• Gene Accession No.: Q9BZS1
• Gene ID (Entrez): 50943
• Immunogen: Full-length human FOXP3 protein
• Primary or Secondary: Primary
• Reconstitution: Protein A purified
• Test Specificity: Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

For Research Use Only