Mouse anti-HIF-1 alpha, Clone: HIF1A-84, Azide Free, Novus Biologicals™

Catalog No.
NBP23463502	0.2mg; Unlabeled

Catalog No. NBP23463502 Supplier Novus Biologicals Supplier No. NBP2346350.2 MG

Description

Mouse Monoclonal Antibody

HIF-1 alpha Monoclonal antibody specifically detects HIF-1 alpha in Human samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Functional, CyTOF-ready.

Specifications

• Antigen: HIF-1 alpha
• Applications: Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Functional Assay, CyTOF
• Classification: Monoclonal
• Clone: HIF1A-84
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Dilution: Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Functional, CyTOF-ready
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Accession No.: Q16665
• Gene Alias: ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
• Gene Symbols: HIF1A
• Host Species: Mouse
• Immunogen: Recombinant human HIF1 alpha protein
• Molecular Weight of Antigen: 93 kDa
• Purification Method: Protein A purified
• Quantity: 0.2 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3091
• Test Specificity: HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
• Reconstitution: Protein A purified
• Target Species: Human
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only