H1F0 Antibody (1415-1), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24518100	Novus Biologicals NBP2451810.02MG	0.02 mg
NBP24518101	Novus Biologicals NBP2451810.1MG	0.1 mg
NBP24518102	Novus Biologicals NBP2451810.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Histone H1 Monoclonal specifically detects Histone H1 in Human, Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Histone H1
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rat
• Gene Accession No.: P07305
• Gene ID (Entrez): 3024
• Immunogen: Nuclei of human leukemia biopsy cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: 1415-1.
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345
• Gene Symbols: H1
• Isotype: IgG2a κ
• Purification Method: Protein A or G purified
• Test Specificity: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

For Research Use Only