Mouse anti-HSP60, Clone: LK2, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2346710.2 MG

Catalog No. NBP23467102

Description

HSP60 Monoclonal antibody specifically detects HSP60 in Human, Mouse, Rat, Bacteria, Chicken, Fungi, Guinea Pig, Hamster, Parasite, Primate, Yeast samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: HSP60
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Gene Accession No.: P10809
• Gene Symbols: HSPD1
• Immunogen: Recombinant human HSP60 protein
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-419 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
• Target Species: Human, Mouse, Rat, Bacteria, Chicken, Fungi, Guinea Pig, Hamster, Parasite, Primate
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)
• Clone: LK2
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: 60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, heat shock 60kD protein 1 (chaperonin), heat shock 60kDa protein 1 (chaperonin), Heat shock protein 60, heat shock protein 65, HLD4, Hsp60, HSP-60, HSP60SPG13, HSP65, HuCHA60, Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, short heat shock protein 60 Hsp60s1, spastic paraplegia 13 (autosomal dominant)
• Host Species: Mouse
• Molecular Weight of Antigen: 60 kDa
• Quantity: 0.2 mg
• Research Discipline: Apoptosis, Cellular Markers, Membrane Trafficking and Chaperones, Mitochondrial Markers
• Gene ID (Entrez): 3329
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only