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Mouse anti-ICAM-3/CD50, Clone: 101-1D2, Novus Biologicals™
SDP

Catalog No. NBP24454902 Shop All R&D Systems Products
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NBP24454902 0.2 mg
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Catalog No. NBP24454902 Supplier Novus Biologicals Supplier No. NBP2445490.2MG

Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

ICAM-3/CD50 Monoclonal antibody specifically detects ICAM-3/CD50 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, SDS-Page, Immunofluorescence.

Specifications

Antigen ICAM-3/CD50
Applications Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Classification Monoclonal
Clone 101-1D2
Concentration 0.2 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias CD50, CD50 antigen, CDW50CDw50, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3
Gene Symbols ICAM3
Host Species Mouse
Immunogen Stimulated human leukocytes
Purification Method Protein A or G purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Cellular Markers, Immunology
Primary or Secondary Primary
Gene ID (Entrez) 3385
Test Specificity Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.
Target Species Human
Content And Storage Store at 4C.
Form Purified
Isotype IgG2a κ
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For Research Use Only

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