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Mouse anti-ICAM-3/CD50, Clone: 101-1D2, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2445490.2MG

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Catalog No. NBP24454902

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Specifications

Specifications

ICAM-3/CD50
101-1D2
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
CD50, CD50 antigen, CDW50CDw50, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3
Mouse
IgG2a Kappa
0.2 mg
Cellular Markers, Immunology
Primary
3385
Human
Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
0.2 mg/mL
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Purified
ICAM3
Stimulated human leukocytes
Protein A or G purified
RUO
Store at 4C.
Monoclonal
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.
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