Mouse anti-ICAM-3/CD50, Clone: 101-1D2, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2445490.2MG

Catalog No. NBP24454902

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

ICAM-3/CD50 Monoclonal antibody specifically detects ICAM-3/CD50 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, SDS-Page, Immunofluorescence.

Specifications

• Antigen: ICAM-3/CD50
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: CD50, CD50 antigen, CDW50CDw50, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Clone: 101-1D2
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: ICAM3
• Immunogen: Stimulated human leukocytes
• Quantity: 0.2 mg
• Research Discipline: Cellular Markers, Immunology
• Gene ID (Entrez): 3385
• Target Species: Human
• Form: Purified

For Research Use Only