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Mouse anti-Kappa Light Chain, Clone: KLC264, Azide Free, Novus Biologicals™
SDP

Catalog No. NBP23465902 Shop All R&D Systems Products
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NBP23465902 0.2 mg
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Catalog No. NBP23465902 Supplier Novus Biologicals Supplier No. NBP2346590.2 MG

Mouse Monoclonal Antibody

Kappa Light Chain Monoclonal antibody specifically detects Kappa Light Chain in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

Antigen Kappa Light Chain
Applications Western Blot, Flow Cytometry, ELISA, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone KLC264
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1:250-1:500, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, CyTOF-ready
Formulation 10mM PBS - BSA free with No Preservative
Gene Accession No. P01601
Gene Alias HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbols IGKC
Host Species Mouse
Immunogen Recombinant human Ig kappa chain
Molecular Weight of Antigen 22.5 kDa
Purification Method Protein A purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Adaptive Immunity, Immunology
Primary or Secondary Primary
Gene ID (Entrez) 3514
Test Specificity This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Reconstitution Protein A purified
Target Species Human
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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