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Mouse anti-Mitochondria, Clone: MTC02, Azide Free, Novus Biologicals™
SDP

Catalog No. NBP23468602 Shop All R&D Systems Products
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0.2 mg
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NBP23468602 0.2 mg
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Catalog No. NBP23468602 Supplier Novus Biologicals Supplier No. NBP2346860.2 MG

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Mouse Monoclonal Antibody

Mitochondria Monoclonal antibody specifically detects Mitochondria in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

Antigen Mitochondria
Applications Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone MTC02
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25-0.5ug/ml, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Formulation 10mM PBS - BSA free with No Preservative
Host Species Mouse
Immunogen Semi-purified mitochondrial preparation
Molecular Weight of Antigen 60 kDa
Purification Method Protein A purified
Quantity 0.2 mg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Reconstitution Protein A purified
Target Species Human, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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