Mouse anti-MS4A1/CD20, Clone: 93-1B3, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2447480.2MG

Catalog No. NBP24474802

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD20 Monoclonal antibody specifically detects CD20 in Human samples. It is validated for Flow Cytometry, Immunofluorescence.

Specifications

• Antigen: CD20
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, Bp35MGC3969, CD20 antigen, CD20 receptor, CD20S7, CVID5, LEU-16, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 1, MS4A2
• Host Species: Mouse
• Molecular Weight of Antigen: 35 kDa
• Quantity: 0.2 mg
• Research Discipline: Adaptive Immunity, Cancer Stem Cells, Cell Biology, Cytokine Research, Immunology, Signal Transduction, Stem Cell Markers, Tumor Biomarkers
• Gene ID (Entrez): 931
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Clone: 93-1B3
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: MS4A1
• Immunogen: Stimulated human leukocytes
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 30-33kDa, which is identified as CD20 (Workshop V; Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only