Mouse anti-MS4A1/CD20, Clone: 93-1B3, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24474802	Novus Biologicals NBP2447480.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD20 Monoclonal antibody specifically detects CD20 in Human samples. It is validated for Flow Cytometry, Immunofluorescence.

Specifications

• Antigen: CD20
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cancer Stem Cells, Cell Biology, Cytokine Research, Immunology, Signal Transduction, Stem Cell Markers, Tumor Biomarkers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 931
• Immunogen: Stimulated human leukocytes
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 35 kDa

• Clone: 93-1B3
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, Bp35MGC3969, CD20 antigen, CD20 receptor, CD20S7, CVID5, LEU-16, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 1, MS4A2
• Gene Symbols: MS4A1
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 30-33kDa, which is identified as CD20 (Workshop V; Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.

For Research Use Only