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  6. Mouse anti-MyoD, Clone: MYD712, Azide Free, Novus Biologicals™

Mouse anti-MyoD, Clone: MYD712, Azide Free, Novus Biologicals™
SDP

Catalog No. NBP23469302 Shop All R&D Systems Products
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0.2 mg
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NBP23469302 0.2 mg
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Catalog No. NBP23469302 Supplier Novus Biologicals Supplier No. NBP2346930.2 MG

Description

Mouse Monoclonal Antibody

MyoD Monoclonal antibody specifically detects MyoD in Human samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

Antigen MyoD
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone MYD712
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
Formulation 10mM PBS - BSA free with No Preservative
Gene Accession No. P15172
Gene Alias bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
Gene Symbols MYOD1
Host Species Mouse
Immunogen Recombinant human MyoD1 protein
Molecular Weight of Antigen 45 kDa
Purification Method Protein A purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Cancer, Cell Cycle and Replication, Growth and Development, Phospho Specific
Primary or Secondary Primary
Gene ID (Entrez) 4654
Test Specificity Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.
Reconstitution Protein A purified
Target Species Human
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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