Mouse anti-NF-H, Clone: RT-97, Novus Biologicals™

Supplier:  Novus Biologicals NBP2294350.2MG

Catalog No. NBP22943502

Specifications

• Antigen: NF-H
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, SDS-Page
• Gene Accession No.: P12036
• Gene Symbols: NEFH
• Immunogen: The Triton-X 100 insoluble protein fraction of rat brain was used as immunogen for the Neurofilament RT-97 antibody.
• Quantity: 0.2 mg
• Research Discipline: Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience
• Gene ID (Entrez): 4744
• Target Species: Human, Mouse, Rat, Pig, Chicken
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), SDS-Page
• Clone: RT-97
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: 200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only