Mouse anti-Siglec-3/CD33, Clone: WM53, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2345110.2 MG

Catalog No. NBP23451102

Description

Siglec-3/CD33 Monoclonal antibody specifically detects Siglec-3/CD33 in Human samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Functional, CyTOF-ready.

Specifications

• Antigen: Siglec-3/CD33
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Frozen 0.5-1ug/ml, Functional, CyTOF-ready
• Gene Accession No.: P20138
• Gene Symbols: CD33
• Immunogen: Human AML cells
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immuno-receptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)
• Clone: WM53
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: CD33 antigen, CD33 antigen (gp67), CD33 molecule, FLJ00391, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, Sialic acid-binding Ig-like lectin 3, SIGLEC-3, SIGLEC3gp67
• Host Species: Mouse
• Molecular Weight of Antigen: 67 kDa
• Quantity: 0.2 mg
• Research Discipline: Immunology
• Gene ID (Entrez): 945
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only