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Mouse anti-Vimentin, Clone: VM452, Novus Biologicals™
SDP

Catalog No. NBP22943302 Shop All R&D Systems Products
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NBP22943302 0.2 mg
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Catalog No. NBP22943302 Supplier Novus Biologicals Supplier No. NBP2294330.2MG

Specifications

Antigen Vimentin
Applications Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone VM452
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, Knockout Validated 0.7 ug/ml
Formulation PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Accession No. P08670
Gene Alias FLJ36605, vimentin
Gene Symbols VIM
Host Species Mouse
Immunogen A recominant protein fragment specific to Vimentin was used as the immunogen for the VM452 antibody.
Purification Method Protein G purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Phospho Specific, Signal Transduction, Stem Cell Markers, Stem Cells
Primary or Secondary Primary
Gene ID (Entrez) 7431
Test Specificity This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Target Species Human, Pig, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG1
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